Difference between revisions of "Part:BBa K143031"

Revision as of 19:59, 16 September 2008

Aad9 Spectinomycin Resistance Gene

Aad9 is the spectinomycin resistance gene from Enterococcus faecalis#1. Expression in a host confers resistance to spectinomycin at a concentration of 100μg/μl and has been used in a variety of vectors for both B. subtilis and E. coli including pDP870#2, pCOMT-Kan#3 and pIEF16s#4

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 488

References

1 pmid=1659306
2 pmid=16997985
3 pmid=15060042
4 pmid=16714443

</biblio>

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K143031 short</partinfo>
 Aad9 is the spectinomycin resistance gene from ''Enterococcus faecalis''<cite>#1</cite>. Expression in a host confers resistance to spectinomycin at a concentration of 100&#956;g/&#956;l and has been used in a variety of vectors for both ''B. subtilis'' and ''E. coli'' including pDP870<cite>#2</cite>, pCOMT-Kan<cite>#3</cite> and pIEF16s<cite>#4</cite>
+<!-- Add more about the biology of this part here
+===Usage and Biology===
+<!-- -->
+<span class='h3bb'>Sequence and Features</span>
+<partinfo>BBa_K143031 SequenceAndFeatures</partinfo>
 ====References====
@@ Line 13: / Line 20: @@
 #4 pmid=16714443
 </biblio>
-<!-- Add more about the biology of this part here
-===Usage and Biology===
-<!-- -->
-<span class='h3bb'>Sequence and Features</span>
-<partinfo>BBa_K143031 SequenceAndFeatures</partinfo>