Difference between revisions of "Part:BBa K3076100:Design"
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Revision as of 13:41, 13 October 2019
No part name specified with partinfo tag.
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Source
The CgMT sequence was identified by a literature review [1] and extracted from NCBI (ACCESSION: KJ638906). Then, codon-optimized for E. coli by the ThermoFisher GeneArt platform.
Design Notes
There was a PstI site at 172 bp to 177 bp after codon optimization, so the codonCTG was changed to CTA which both codon codes for leucine. When synthesized into the expression vector, the start codon of this part was removed because the vector contains its own start codon.
References
[1] Jafarian, V., & Ghaffari, F. (2017). A unique metallothionein-engineered in Escherichia coli for biosorption of lead, zinc, and cadmium; absorption or adsorption? Microbiology, 86(1), 73–81. doi: 10.1134/s0026261717010064