Difference between revisions of "Part:BBa K3286003"

 
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<partinfo>BBa_K3286003 short</partinfo>
 
<partinfo>BBa_K3286003 short</partinfo>
  
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Expression cassette of the sgRNA with non-targeting (NT) spacer (https://parts.igem.org/Part:BBa_K3286001) and rho-independent terminator (https://parts.igem.org/Part:BBa_K3286002) under control of the strong constitutive Anderson promoter J23109 (https://parts.igem.org/Part:BBa_J23119).  
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Expression cassette of the sgRNA for Cas9 of <i>Streptococcus pyogenes</i> with non-targeting (NT) spacer (https://parts.igem.org/Part:BBa_K3286001) and rho-independent terminator (https://parts.igem.org/Part:BBa_K3286002) under control of the strong constitutive Anderson promoter J23109 (https://parts.igem.org/Part:BBa_J23119).  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 13:38, 13 October 2019


sgRNA expression module

Expression cassette of the sgRNA for Cas9 of Streptococcus pyogenes with non-targeting (NT) spacer (https://parts.igem.org/Part:BBa_K3286001) and rho-independent terminator (https://parts.igem.org/Part:BBa_K3286002) under control of the strong constitutive Anderson promoter J23109 (https://parts.igem.org/Part:BBa_J23119).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]