Difference between revisions of "Part:BBa K3137001"
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<partinfo>BBa_K3137001 short</partinfo>
 
<partinfo>BBa_K3137001 short</partinfo>
  
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AbpAB are two genes of the E.coli genome that express resistant proteins that are resistant to T2,T4,T7 and λ phages. We obtained abpA and abpB by PCR from the genome of E.coli BL21 and constructed recombinant plasmid that connected abpA and abpB at the same time, then used IPTG to induce the expression of resistant protein and verified the resistant function of the protein.
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DnaE is the alpha subunit of DNA polymerase III which catalyzes the polymerase activity of the holoenzyme complex.
  
 
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Revision as of 13:28, 13 October 2019


Improved RBS 4

DnaE is the alpha subunit of DNA polymerase III which catalyzes the polymerase activity of the holoenzyme complex.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]