Difference between revisions of "Part:BBa K3137001:Design"
(→References) |
(→Design Notes) |
||
| Line 8: | Line 8: | ||
===Design Notes=== | ===Design Notes=== | ||
The coding sequences of abpA and abpB overlap by 4 bp, which may facilitate the coupling of their expression at the translational level. | The coding sequences of abpA and abpB overlap by 4 bp, which may facilitate the coupling of their expression at the translational level. | ||
| − | |||
| − | |||
===Source=== | ===Source=== | ||
Revision as of 13:23, 13 October 2019
Improved RBS 4
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The coding sequences of abpA and abpB overlap by 4 bp, which may facilitate the coupling of their expression at the translational level.
Source
abpA and abpB are obtained by PCR from the genome of E.coli BL21.
References
Yasui R , Washizaki A , Furihata Y , et al. AbpA and AbpB provide anti-phage activity in Escherichia coli[J]. Genes & Genetic Systems, 2014, 89(2):51-60.