Difference between revisions of "Part:BBa K3076300:Design"
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<partinfo>BBa_K3076300 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3076300 SequenceAndFeatures</partinfo> | ||
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===Source=== | ===Source=== |
Revision as of 13:17, 13 October 2019
dsDNA substrate with KanR gene for CopA knockout in E. coli by Lambda Red Recombineering
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Source
The genomic sequence of CopA gene was extracted from NCBI (ACCESSION: NC_000913 REGION: complement (508875..511379)). The kanamycin resistance gene was extracted from the sequence of pET28a plasmid sequence from addgene.org. Double terminator was extracted from iGEM part registry (BBa B0015) which is the most commonly used double terminator with high efficiency.
Design Notes
The recombination site was chosen to be near the gene's 5' end of the coding strand to increase the chance of successful knockout.