Difference between revisions of "Part:BBa K2947002"
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As shown in figure.1 and figure.2, the expression of protoplasts showed that the fluorescence intensity of GFP regulated by d35S promoter was stronger than that regulated by 35S promoter. | As shown in figure.1 and figure.2, the expression of protoplasts showed that the fluorescence intensity of GFP regulated by d35S promoter was stronger than that regulated by 35S promoter. | ||
| − | <html><img src="https://2019.igem.org/wiki/images/4/4b/T--GDSYZX--K2947002_P3.jpg" width=" | + | <html><img src="https://2019.igem.org/wiki/images/4/4b/T--GDSYZX--K2947002_P3.jpg" width="700" height="400" BORDER=0</ a></html> |
figure.3 | figure.3 | ||
Latest revision as of 12:53, 13 October 2019
d35S
d35S refers to Double GoldenGate compatible 35S Promoter.
Usage and Biology
35S is a plant specific promoter obtained from the Cauliflower Mosaic Virus.
We add two 35s promotor together to get a d35s promotor, then we merge GFP gene with d35s promotor to make a binary expression vector. After that, we extract plasmids, transfer the plasmids into the protoplasts of Arabidopsis thaliana and culture the protoplasts for 12 hours to view fluorescence intensity run protein gel electrophoresis and Western blot to test the GFP expression rate.
figure.1
figure.2
As shown in figure.1 and figure.2, the expression of protoplasts showed that the fluorescence intensity of GFP regulated by d35S promoter was stronger than that regulated by 35S promoter.
figure.3
As shown in figure.3, the protoplast protein expression experiment showed that the expression of GFP under the regulation of d35S promoter was higher than that under the regulation of 35S promoter. Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]