Difference between revisions of "Part:BBa K2947002"
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<partinfo>BBa_K2947002 short</partinfo>
 
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d35S refers to Double GoldenGate compatible 35S Promoter. 35S is a plant specific promoter obtained from the Cauliflower Mosaic Virus.
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d35S refers to Double GoldenGate compatible 35S Promoter.  
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We add two 35s promotor together to get a d35s promotor, then we merge GFP gene with d35s promotor to make a binary expression vector. After that, we extract plasmids, transfer the plasmids into the protoplasts of Arabidopsis thaliana and culture the protoplasts for 12 hours to view fluorescence intensity run protein gel electrophoresis and Western blot to test the GFP expression rate.
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===Usage and Biology===
 
===Usage and Biology===
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35S is a plant specific promoter obtained from the Cauliflower Mosaic Virus.
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 +
We add two 35s promotor together to get a d35s promotor, then we merge GFP gene with d35s promotor to make a binary expression vector. After that, we extract plasmids, transfer the plasmids into the protoplasts of Arabidopsis thaliana and culture the protoplasts for 12 hours to view fluorescence intensity run protein gel electrophoresis and Western blot to test the GFP expression rate.
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<html><img src="https://2019.igem.org/wiki/images/9/9f/T--GDSYZX--K2947002_P1.jpg" width="400" height="400" BORDER=0</ a></html>
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figure.1
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<html><img src="https://2019.igem.org/wiki/images/f/f0/T--GDSYZX--K2947002_P2.jpg" width="400" height="400" BORDER=0</ a></html>
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figure.2
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As shown in figure.1 and figure.2, the expression of protoplasts showed that the fluorescence intensity of GFP regulated by d35S promoter was stronger than that regulated by 35S promoter.
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<html><img src="https://2019.igem.org/wiki/images/4/4b/T--GDSYZX--K2947002_P3.jpg" width="400" height="400" BORDER=0</ a></html>
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figure.3
  
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As shown in figure.3, the protoplast protein expression experiment showed that the expression of GFP under the regulation of d35S promoter was higher than that under the regulation of 35S promoter.
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 12:51, 13 October 2019


d35S

d35S refers to Double GoldenGate compatible 35S Promoter.


Usage and Biology

35S is a plant specific promoter obtained from the Cauliflower Mosaic Virus.

We add two 35s promotor together to get a d35s promotor, then we merge GFP gene with d35s promotor to make a binary expression vector. After that, we extract plasmids, transfer the plasmids into the protoplasts of Arabidopsis thaliana and culture the protoplasts for 12 hours to view fluorescence intensity run protein gel electrophoresis and Western blot to test the GFP expression rate.

figure.1 figure.2

As shown in figure.1 and figure.2, the expression of protoplasts showed that the fluorescence intensity of GFP regulated by d35S promoter was stronger than that regulated by 35S promoter.

figure.3

As shown in figure.3, the protoplast protein expression experiment showed that the expression of GFP under the regulation of d35S promoter was higher than that under the regulation of 35S promoter. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]