Difference between revisions of "Part:BBa K143020:Design"

 
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===References===
 
===References===
 +
<biblio>
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#1 pmid=9669336
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#2 pmid=6181373
 +
</biblio>

Revision as of 22:30, 15 September 2008

GsiB ribosome binding site (RBS) for B. subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to ensure that the RBS is functional the actual ribosome binding site was maintained and the distance between the RBS and the start codon maintained. In order to conform to the biobrick standard the sequence flanking the RBS had to be changed but the distance between the promoter and RBS, and start codon and RBS was maintained.



Source

The sequence was taken from a previous research paper [1] and was constructed by Geneart

References

<biblio>

  1. 1 pmid=9669336
  2. 2 pmid=6181373

</biblio>