Difference between revisions of "Part:BBa K3113302"

Revision as of 11:51, 13 October 2019

pCAG_Gag-HiBiT-L7Ae

This plasmid codes for the coat protein of HIV fused to a split luciferase fused to a synthetic L7Ae. This construct forms virus-like particles which can be detected via a split luciferase assay. It is used to target fluc specifically into exosomes via L7Ae-C/Dbox connection.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 3019
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2319

Revision as of 11:36, 12 October 2019 (view source) Registry (Talk \| contribs)		Revision as of 11:51, 13 October 2019 (view source) Theresakeil (Talk \| contribs) Newer edit →
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	<partinfo>BBa_K3113302 short</partinfo>		<partinfo>BBa_K3113302 short</partinfo>

−	This plasmid codes for the coat protein of ~~gag~~ fused to a split luciferase fused to a synthetic L7Ae. This construct forms virus-like particles which can be detected via a split luciferase assay. It is used to target fluc specifically into exosomes via L7Ae-C/Dbox connection.	+	This plasmid codes for the coat protein of HIV fused to a split luciferase fused to a synthetic L7Ae. This construct forms virus-like particles which can be detected via a split luciferase assay. It is used to target fluc specifically into exosomes via L7Ae-C/Dbox connection.