Difference between revisions of "Part:BBa K3002017"

Revision as of 10:32, 13 October 2019

3xHA tag for Chlamydomonas reinhardtii (Phytobrick)

This basic part contains the sequence of the 3xHA-tag (B5) and was built as a part of the Kaiser Collection. Combined with a promoter (e.g. BBa_K3002001 (PSAD promoter (A1-B1)) or BBa_K3002031 (PAR promoter (A1-B1))) and terminator (e.g. BBa_K3002006 (RPL23 terminator)) your target protein is secreted efficiently and can be detected via HA-antibody.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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-This basic part contains the sequence of the HA-tag (B5) and was built as a part of the Kaiser Collection. Combined with a promoter (e.g. <a href="https://parts.igem.org/Part:BBa_K3002001">BBa_K3002001</a> (PSAD promoter (A1-B1)) or <a href="https://parts.igem.org/Part:BBa_K3002031">BBa_K3002031</a> (PAR promoter (A1-B1))) and terminator (e.g. <a href="https://parts.igem.org/Part:BBa_K3002006">BBa_K3002006</a> (RPL23 terminator)) your target protein is secreted efficiently and can be detected via HA-antibody.
+This basic part contains the sequence of the 3xHA-tag (B5) and was built as a part of the Kaiser Collection. Combined with a promoter (e.g. <a href="https://parts.igem.org/Part:BBa_K3002001">BBa_K3002001</a> (PSAD promoter (A1-B1)) or <a href="https://parts.igem.org/Part:BBa_K3002031">BBa_K3002031</a> (PAR promoter (A1-B1))) and terminator (e.g. <a href="https://parts.igem.org/Part:BBa_K3002006">BBa_K3002006</a> (RPL23 terminator)) your target protein is secreted efficiently and can be detected via HA-antibody.
 </html>