Difference between revisions of "Part:BBa K3257076:Design"
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
The tRNA primer is almost the same as the tRNA<sup>pro</sup> in bacteria cells while the last 18 nucleotides of  3' terminal of the primer are replaced by the last 18 nucleotides of  3' terminal of the gene of interest. Our software can help with the design of the user-specific tRNA primer. Please refer to the software page of 2019 Fudan-TSI wiki (https://2019.igem.org/Team:Fudan-TSI/Software).
+
The tRNA primer is almost the same as the tRNA<sup>pro</sup> in bacteria cells while the last 18 nucleotides of  3' terminal of the primer are replaced by the last 18 nucleotides of  3' terminal of the gene of interest. Our software can help with the design of the user-specific tRNA primer. Please refer to the software page of 2019 Fudan-TSI wiki ( https://2019.igem.org/Team:Fudan-TSI/Software ).
  
  

Latest revision as of 09:28, 13 October 2019


tRNA Primer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The tRNA primer is almost the same as the tRNApro in bacteria cells while the last 18 nucleotides of 3' terminal of the primer are replaced by the last 18 nucleotides of 3' terminal of the gene of interest. Our software can help with the design of the user-specific tRNA primer. Please refer to the software page of 2019 Fudan-TSI wiki ( https://2019.igem.org/Team:Fudan-TSI/Software ).


Source

Artificially synthesized.

References