Difference between revisions of "Part:BBa K3205002"
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AreR is the regulatory protein of Acinetobacter sp. Strain ADP1. "are" operon and can be activated by benzyl alcohol or other aromatic compounds such as benzyl acetate, benzaldehyde, 2- and 4-hydroxybenzyl acetates, benzyl propionate and butyrate.
 
AreR is the regulatory protein of Acinetobacter sp. Strain ADP1. "are" operon and can be activated by benzyl alcohol or other aromatic compounds such as benzyl acetate, benzaldehyde, 2- and 4-hydroxybenzyl acetates, benzyl propionate and butyrate.
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Revision as of 01:44, 13 October 2019


AreR_Acinetobacter sp. Strain ADP1

AreR is the regulatory protein of Acinetobacter sp. Strain ADP1. "are" operon and can be activated by benzyl alcohol or other aromatic compounds such as benzyl acetate, benzaldehyde, 2- and 4-hydroxybenzyl acetates, benzyl propionate and butyrate.


Usage and Biology

AreR is a member of the NtrC/XylR regulatory protein family and controls the expression of the areCBA genes in Acinetobacter sp. Strain ADP1. This σ54-type enhancer protein can bind to the repeat sequence upstream of the Pare promoter after activated by benzyl alcohol. In the case of cofactor-induced DNA looping, a closed promoter complex will be formed with RNA polymerase to turn on the downstream genes transcription. This protein can form a complete gene expression regulator with the Pare promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5
    Illegal BglII site found at 651
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 612
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

For more details, please click here https://parts.igem.org/Part:BBa_K3205000