Difference between revisions of "Part:BBa K3205002"

Revision as of 01:25, 13 October 2019

AreR_Acinetobacter sp. Strain ADP1

AreR is the regulatory protein of Acinetobacter sp. Strain ADP1. "are" operon and can be activated by benzyl alcohol or other aromatic compounds such as benzyl acetate, benzaldehyde, 2- and 4-hydroxybenzyl acetates, benzyl propionate and butyrate.

Usage and Biology

AreR is a member of the NtrC/XylR regulatory protein family and controls the expression of the areCBA genes in Acinetobacter sp. Strain ADP1. This σ54-type enhancer protein can bind to the repeat sequence upstream of the Pare promoter after activated by benzyl alcohol. In the case of cofactor-induced DNA looping, a closed promoter complex will be formed with RNA polymerase to turn on the downstream genes transcription. This protein can form a complete gene expression regulator with the Pare promoter.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 5
Illegal BglII site found at 651
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 612
1000
COMPATIBLE WITH RFC[1000]

Functional Parameters

For more details, please click here https://parts.igem.org/Part:BBa_K3205000

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3205002 short</partinfo>
-AreR is a member of the NtrC/XylR regulatory protein family, controls the expression of the areCBA genes in Acinetobacter sp. Strain ADP1. This &#963;^54-type enhancer protein can binding to the repeat sequence upstream of the Pare promoter after activated by benzyl alcohol or other aromatic compounds such as benzyl acetate, benzaldehyde, as well as 2- and 4-hydroxybenzyl acetates and benzyl propionate and butyrate. In the case of cofactor-induced DNA looping, a closed promoter complex will be formed with RNA polymerase to turn on the downstream gene transcription.
+AreR is the regulatory protein of Acinetobacter sp. Strain ADP1. "are" operon and can be activated by benzyl alcohol or other aromatic compounds such as benzyl acetate, benzaldehyde, 2- and 4-hydroxybenzyl acetates, benzyl propionate and butyrate.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+AreR is a member of the NtrC/XylR regulatory protein family and controls the expression of the areCBA genes in Acinetobacter sp. Strain ADP1. This σ54-type enhancer protein can bind to the repeat sequence upstream of the Pare promoter after activated by benzyl alcohol. In the case of cofactor-induced DNA looping, a closed promoter complex will be formed with RNA polymerase to turn on the downstream genes transcription. This protein can form a complete gene expression regulator with the Pare promoter.
 <!-- -->
@@ Line 15: / Line 18: @@
 ===Functional Parameters===
+For more details, please click here
 https://parts.igem.org/Part:BBa_K3205000
 <partinfo>BBa_K3205002 parameters</partinfo>
 <!-- -->