Difference between revisions of "Part:BBa K3205001"
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<partinfo>BBa_K3205001 short</partinfo>
 
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Pare is a AreR-regulated &#963;^54-dependent promoter in Acinetobacter sp. Strain ADP1. AreR can be activated by benzyl alcohol or other aromatic compounds such as benzyl acetate, benzaldehyde, 2- and 4-hydroxybenzyl acetates, benzyl propionate and butyrate. In the case of cofactor-induced DNA looping, a closed promoter complex will be formed with RNA polymerase to turn on the downstream gene transcription.
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AreR is the regulatory protein of Acinetobacter sp. Strain ADP1. "are" operon and can be activated by benzyl alcohol or other aromatic compounds such as benzyl acetate, benzaldehyde, 2- and 4-hydroxybenzyl acetates, benzyl propionate and butyrate.
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===Usage and Biology===
 
===Usage and Biology===
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AreR is a member of the NtrC/XylR regulatory protein family and controls the expression of the areCBA genes in Acinetobacter sp. Strain ADP1. This σ54-type enhancer protein can bind to the repeat sequence upstream of the Pare promoter after activated by benzyl alcohol. In the case of cofactor-induced DNA looping, a closed promoter complex will be formed with RNA polymerase to turn on the downstream genes transcription. This protein can form a complete gene expression regulator with the Pare promoter.
  
 
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===Functional Parameters===
 
===Functional Parameters===
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For more details, click here
 
https://parts.igem.org/Part:BBa_K3205000
 
https://parts.igem.org/Part:BBa_K3205000
 
<partinfo>BBa_K3205001 parameters</partinfo>
 
<partinfo>BBa_K3205001 parameters</partinfo>
 
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Revision as of 00:51, 13 October 2019


Pare promoter region

AreR is the regulatory protein of Acinetobacter sp. Strain ADP1. "are" operon and can be activated by benzyl alcohol or other aromatic compounds such as benzyl acetate, benzaldehyde, 2- and 4-hydroxybenzyl acetates, benzyl propionate and butyrate.


Usage and Biology

AreR is a member of the NtrC/XylR regulatory protein family and controls the expression of the areCBA genes in Acinetobacter sp. Strain ADP1. This σ54-type enhancer protein can bind to the repeat sequence upstream of the Pare promoter after activated by benzyl alcohol. In the case of cofactor-induced DNA looping, a closed promoter complex will be formed with RNA polymerase to turn on the downstream genes transcription. This protein can form a complete gene expression regulator with the Pare promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

For more details, click here https://parts.igem.org/Part:BBa_K3205000