Difference between revisions of "Part:BBa K2983060:Design"
TomZaplana (Talk | contribs) |
TomZaplana (Talk | contribs) |
||
| Line 21: | Line 21: | ||
DNA synthesis | DNA synthesis | ||
| − | |||
| − | |||
Latest revision as of 23:31, 12 October 2019
RedStar2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Derived from the Addgene plasmid #120779.
The 3 EcoRI sites (GAATTC) were changed to GAgTTC.
The 3 BbsI sites (GAAGAC) were changed to GAgGAC or GAAaAC.
The BsmBI site (CGTCTC) was changed to CaTCTC.
All these mutations are synonymous.
Source
DNA synthesis