Difference between revisions of "Part:BBa K2983053"

 
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__NOTOC__
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<partinfo>BBa_K2983053 short</partinfo>
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This part is a version of the ''Yarrowia lipolytica'' pTef1 promoter, derived from the wild type pTef1 promoter (nucleotides 1227367 to 1227009) of ''Yarrowia lipolytica'' W29 chromosome C (GenBank Acc. n° CP028450.1). Four additional nucleotides (CACA) are present at the end of this part in order to be directly upstream of the ATG of a gene placed under its control.
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It is an improvement of [[Part:BBa_K2117000|BBa_K2117000]] as it is shorter and does not have the BsaI site which makes this part compatible with the iGEM Type IIS RFC[1000] standard.
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2983053 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K2983053 parameters</partinfo>
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Revision as of 22:26, 12 October 2019


pTef1f: wild-type without the BsaI site, shorter and with CACA before ATG

This part is a version of the Yarrowia lipolytica pTef1 promoter, derived from the wild type pTef1 promoter (nucleotides 1227367 to 1227009) of Yarrowia lipolytica W29 chromosome C (GenBank Acc. n° CP028450.1). Four additional nucleotides (CACA) are present at the end of this part in order to be directly upstream of the ATG of a gene placed under its control.

It is an improvement of BBa_K2117000 as it is shorter and does not have the BsaI site which makes this part compatible with the iGEM Type IIS RFC[1000] standard.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 192
    Illegal PstI site found at 157
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 192
    Illegal PstI site found at 157
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 192
    Illegal PstI site found at 157
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 192
    Illegal PstI site found at 157
  • 1000
    COMPATIBLE WITH RFC[1000]