Difference between revisions of "Part:BBa K2983051:Design"

 
 
Line 1: Line 1:
  
 +
__NOTOC__
 +
<partinfo>BBa_K2983051 short</partinfo>
 +
 +
<partinfo>BBa_K2983051 SequenceAndFeatures</partinfo>
 +
 +
 +
===Design Notes===
 +
Nucleotides 1227374 to 1226969 of ''Yarrowia lipolytica'' W29 chromosome C (GenBank Acc. n° CP028450.1) with the BsaI site (GGTCTC) changed to GGTCTg.
 +
 +
 +
===Source===
 +
 +
DNA synthesis
 +
 +
===References===

Latest revision as of 22:00, 12 October 2019


pTef1d: wild-type without the BsaI site


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 199
    Illegal PstI site found at 164
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 199
    Illegal PstI site found at 164
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 199
    Illegal PstI site found at 164
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 199
    Illegal PstI site found at 164
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Nucleotides 1227374 to 1226969 of Yarrowia lipolytica W29 chromosome C (GenBank Acc. n° CP028450.1) with the BsaI site (GGTCTC) changed to GGTCTg.


Source

DNA synthesis

References