Difference between revisions of "Part:BBa K2983051:Design"
TomZaplana (Talk | contribs) |
|||
Line 1: | Line 1: | ||
+ | __NOTOC__ | ||
+ | <partinfo>BBa_K2983051 short</partinfo> | ||
+ | |||
+ | <partinfo>BBa_K2983051 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | |||
+ | ===Design Notes=== | ||
+ | Nucleotides 1227374 to 1226969 of ''Yarrowia lipolytica'' W29 chromosome C (GenBank Acc. n° CP028450.1) with the BsaI site (GGTCTC) changed to GGTCTg. | ||
+ | |||
+ | |||
+ | ===Source=== | ||
+ | |||
+ | DNA synthesis | ||
+ | |||
+ | ===References=== |
Latest revision as of 22:00, 12 October 2019
pTef1d: wild-type without the BsaI site
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 199
Illegal PstI site found at 164 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 199
Illegal PstI site found at 164 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 199
Illegal PstI site found at 164 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 199
Illegal PstI site found at 164 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Nucleotides 1227374 to 1226969 of Yarrowia lipolytica W29 chromosome C (GenBank Acc. n° CP028450.1) with the BsaI site (GGTCTC) changed to GGTCTg.
Source
DNA synthesis