Difference between revisions of "Part:BBa K3002004:Design"

 
 
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===Source===
 
===Source===
  
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This part originates from Ideonella sakaiensis (Yoshida, S., 2016).
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This part originates from Ideonella sakaiensis (<a href="https://science.sciencemag.org/content/351/6278/1196.full">Yoshida, S., 2016</a>).
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===References===
 
===References===

Latest revision as of 21:49, 12 October 2019


Wildtype PETase for Chlamydomonas reinhardtii (Phytobrick)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 507
    Illegal PstI site found at 1480
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 507
    Illegal PstI site found at 1480
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 507
    Illegal PstI site found at 1480
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 507
    Illegal PstI site found at 1480
    Illegal NgoMIV site found at 242
    Illegal NgoMIV site found at 269
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The natural secretion signal of Ideonella sakaiensis was removed. This part is codon-optimized for Chlamydomonas reinhardtii. Additionally, it is compatible with Phytobricks /MoClo parts.


Source

This part originates from Ideonella sakaiensis (Yoshida, S., 2016).

References