Difference between revisions of "Part:BBa K2923001"

Line 6: Line 6:
 
Theophylline (1,3-dimethylxanthine) is a drug used in therapy for respiratory diseases and is structurally similar to theobromine and caffeine.  
 
Theophylline (1,3-dimethylxanthine) is a drug used in therapy for respiratory diseases and is structurally similar to theobromine and caffeine.  
 
Aptazyme is composed in 3 different parts, a twister ribozyme module, a communication module, and an aptamer module. The described anti-theophylline aptazyme is an OFF-switch. Its catalytic activity (self-cleavage) is related to ligand absence.  
 
Aptazyme is composed in 3 different parts, a twister ribozyme module, a communication module, and an aptamer module. The described anti-theophylline aptazyme is an OFF-switch. Its catalytic activity (self-cleavage) is related to ligand absence.  
 +
 +
The no highlighted sequences correspond to the riboswitch module.
  
  

Revision as of 21:47, 12 October 2019


Theophylline actif aptazyme

An aptazyme is an aptamer-ribozyme combination with intrinsic RNA cleavage activity. This aptazyme sequence is specific for the theophylline (ligand). Theophylline (1,3-dimethylxanthine) is a drug used in therapy for respiratory diseases and is structurally similar to theobromine and caffeine. Aptazyme is composed in 3 different parts, a twister ribozyme module, a communication module, and an aptamer module. The described anti-theophylline aptazyme is an OFF-switch. Its catalytic activity (self-cleavage) is related to ligand absence.

The no highlighted sequences correspond to the riboswitch module.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]