Difference between revisions of "Part:BBa K2923001"

 
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<partinfo>BBa_K2923001 short</partinfo>
 
<partinfo>BBa_K2923001 short</partinfo>
  
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an aptazyme is an aptamer-ribozyme combinations with intrinsic RNA cleavage activity.  
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An aptazyme is an aptamer-ribozyme combination with intrinsic RNA cleavage activity. This aptazyme sequence is specific for the theophylline (ligand).  
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this aptazyme is specific for the theophylline (liguant). Theophylline (1,3-dimethylxanthine) is a drug used in therapy for respiratory diseases and is structurally similar to theobromine and caffeine
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Theophylline (1,3-dimethylxanthine) is a drug used in therapy for respiratory diseases and is structurally similar to theobromine and caffeine.
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He is composed in 3 differents part, a twister ribozyme module, a communication module and an aptamer module
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Aptazyme is composed in 3 different parts, a twister ribozyme module, a communication module, and an aptamer module. The described anti-theophylline aptazyme is an OFF-switch. Its catalytic activity (self-cleavage) is related to ligand absence.  
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This aptazyme is a OFF-switches aptazyme, this mean that in the presence of liguant the RNA does not self-cleave and in the absence of liguant the RNA self-cleaves.
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This mean that you can sense the theophylline in a medium with this aptazyme.
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Revision as of 21:34, 12 October 2019


Theophylline actif aptazyme

An aptazyme is an aptamer-ribozyme combination with intrinsic RNA cleavage activity. This aptazyme sequence is specific for the theophylline (ligand). Theophylline (1,3-dimethylxanthine) is a drug used in therapy for respiratory diseases and is structurally similar to theobromine and caffeine. Aptazyme is composed in 3 different parts, a twister ribozyme module, a communication module, and an aptamer module. The described anti-theophylline aptazyme is an OFF-switch. Its catalytic activity (self-cleavage) is related to ligand absence.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]