Difference between revisions of "Part:BBa K2959010:Design"

 
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===References===
 
===References===
 +
1. Christensen, H. E. M., Ramachandran, S., Tan, C.-T., Surana, U., Dong, C.-H., & Chua, N.-H. (1996). Arabidopsis profilins are functionally similar to yeast profilins: identification of a vascular bundle-specific profilin and a pollen-specific profilin. The Plant Journal, 10(2), 269–279. doi:10.1046/j.1365-313x.1996.10020269.x

Latest revision as of 20:53, 12 October 2019


Expressible Arabidopsis thaliana Profilin 1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence of AtPFN1 is succeeded by a 6x His-Tag to facilitate its purification by immobilized metal affinity chromatography.



Source

The composite part was designed using the following parts previously added to the Registry: BBa_K525998, and BBa_B0015. The amino acid sequence for AtPFN1 was retrieved from Christensen et al. (1996).


References

1. Christensen, H. E. M., Ramachandran, S., Tan, C.-T., Surana, U., Dong, C.-H., & Chua, N.-H. (1996). Arabidopsis profilins are functionally similar to yeast profilins: identification of a vascular bundle-specific profilin and a pollen-specific profilin. The Plant Journal, 10(2), 269–279. doi:10.1046/j.1365-313x.1996.10020269.x