Difference between revisions of "Part:BBa K3037001:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
Tagging (removable tag)
+
Prescission site upstream and downstream.
−
Affinity chromatography Purification
+
−
Improvement of yield of recombinant proteins (increase expression and increase solubility)
+
−
Improvement of solubility of recombinant proteins (solving problem of inclusion bodies)
+
−
Can help expression of difficult proteins like Cas9 and fusion proteins
+
  
 +
Biobrick used in RFC 25 standard
  
 +
Codon optimized for <span style="font-style: italic;">E. coli</span> with all forbidden restriction enzyme sites removed
  
  

Revision as of 18:59, 12 October 2019


Maltose Binding Protein (MBP-tag)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 381
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 79


Design Notes

Prescission site upstream and downstream.

Biobrick used in RFC 25 standard

Codon optimized for E. coli with all forbidden restriction enzyme sites removed


Source

Synthesized by Integrated DNA Technologies (IDT)

References