Difference between revisions of "Part:BBa K2951000"
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==Characterizaiton==
 
==Characterizaiton==
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This part was characterized by CSMU_Taiwan 2019.Because of its stringent sequence specificity, tobacco etch virus (TEV) protease emerges as a useful reagent with wide application in the cleavage of recombinant fusion proteins. Thus, we have chosen TEV protease(sequence from [https://parts.igem.org/Part:BBa_K1319004 BBa_K1319004]) to remove the fusion protein in our another part [https://parts.igem.org/Part:BBa_K2951008 BBa_K2951008] and be characterized.
 
  
 
===Small scale production===
 
===Small scale production===

Revision as of 18:14, 12 October 2019

Influenza A virus nucleocapsid protein

Short Description

This part contains the original sequence of Influenza A nucleocapsid protein(NP) obtained from NCBI.

Characterizaiton

Small scale production

Cultivations and Induction of protein expression

Large scale production

Cultivations and Induction of protein expression

Protein solubility analysis

Protein purification and dialysis

References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 477
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 238