Difference between revisions of "Part:BBa K3102014"
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E.coli, lldP is an inner membrane permease that functions in the uptake of L-lactate, D-lactate and glycolate [Dong et al., 1993; Nunez et al., 2002]. The lldP gene is located in a lactate-inducible operon with the lldD and lldR genes encoding a lactate dehydrogenase and a regulatory protein, respectively. By overexpressing a plasmid carrying this enzyme, the bacteria is able to uptake the double of these three substrates, L-lactate, D-lactate and glycolate.  
 
E.coli, lldP is an inner membrane permease that functions in the uptake of L-lactate, D-lactate and glycolate [Dong et al., 1993; Nunez et al., 2002]. The lldP gene is located in a lactate-inducible operon with the lldD and lldR genes encoding a lactate dehydrogenase and a regulatory protein, respectively. By overexpressing a plasmid carrying this enzyme, the bacteria is able to uptake the double of these three substrates, L-lactate, D-lactate and glycolate.  
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Dong JM et al., Three overlapping lct genes involved in L-lactate utilization by Escherichia coli., Journal of Bacteriology, 1993 Oct;175(20):6671-8.
 
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Nunez MF et al., Transport of L-Lactate, D-Lactate, and glycolate by the LldP and GlcA membrane carriers of Escherichia coli, Biochem Biophys Res Commun. 2002 Jan 18;290(2):824-9.
 
  
 
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Revision as of 17:02, 12 October 2019


LldP (L-lactate permease)

E.coli, lldP is an inner membrane permease that functions in the uptake of L-lactate, D-lactate and glycolate [Dong et al., 1993; Nunez et al., 2002]. The lldP gene is located in a lactate-inducible operon with the lldD and lldR genes encoding a lactate dehydrogenase and a regulatory protein, respectively. By overexpressing a plasmid carrying this enzyme, the bacteria is able to uptake the double of these three substrates, L-lactate, D-lactate and glycolate.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 275
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 411
    Illegal AgeI site found at 874
    Illegal AgeI site found at 1312
    Illegal AgeI site found at 1471
    Illegal AgeI site found at 1483
  • 1000
    COMPATIBLE WITH RFC[1000]