Difference between revisions of "Part:BBa K3102011:Design"

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===References===
 
===References===
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"Homology between cellulase genes of Trichoderma reesei: complete nucleotide sequence of the endoglucanase I gene." Penttilae M., Lehtovaara P., Nevalainen H., Bhikhabhai R., Knowles J.K.C. Gene 45:253-263(1986)
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Moriyoshi K, Ohmoto T, Ohe T, Sakai K. Role of Endo-1,4-β-glucanases from Neisseria sicca SB in synergistic degradation of cellulose acetate. Biosci Biotechnol Biochem. 2003;67(2):250–7.

Revision as of 16:48, 12 October 2019


EG1 (Endo-1,4-beta-glucanase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1112
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 55
    Illegal AgeI site found at 313
    Illegal AgeI site found at 403
    Illegal AgeI site found at 1240
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence synthesized and optimized for E.coli on Geneius



Source

Neisseria Sicca, sequence found through genebank https://www.ncbi.nlm.nih.gov/protein/BAJ21449.1

References

Moriyoshi K, Ohmoto T, Ohe T, Sakai K. Role of Endo-1,4-β-glucanases from Neisseria sicca SB in synergistic degradation of cellulose acetate. Biosci Biotechnol Biochem. 2003;67(2):250–7.