Difference between revisions of "Part:BBa K2985002"

Revision as of 16:39, 12 October 2019

yerP->express self-resistence of surfactin

YerP gene plays an important role in surfactin production in bacterial cells. According to the information of many literatures, although the specific working principle of yerP gene has not been found yet, the current statement is that gene yerP encodes a specific resistance protein, so that bacterial cells can still grow at a higher surfactin concentration. At the same time, in the presence of yerP, the production of surfactin has been greatly improved, but for cell growth and Producing surfactin is not a necessary gene.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 408
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 112
Illegal NgoMIV site found at 2965
Illegal AgeI site found at 237
Illegal AgeI site found at 1627
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 2093

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 <partinfo>BBa_K2985002 short</partinfo>
-The lia gene cluster is mainly involved in the encoding of lia protein, which can protect the integrity of cell membrane. Meanwhile, liaH and liaI exist in lia large gene cluster, and the two-component system liaRS can effectively resist the damage of lipids and peptides like surfactin on cells. It can enhance the resistance of bacteria to lipopeptide antibiotics.
+YerP gene plays an important role in surfactin production in bacterial cells. According to the information of many literatures, although the specific working principle of yerP gene has not been found yet, the current statement is that gene yerP encodes a specific resistance protein, so that bacterial cells can still grow at a higher surfactin concentration. At the same time, in the presence of yerP, the production of surfactin has been greatly improved, but for cell growth and Producing surfactin is not a necessary gene.
-At the same time, these two genes only exist in the lia large gene cluster, and half of them exist in bacillus subtilis.
-Using these two genes, the sequence and template can be obtained from bacillus subtilis, and the gene can be obtained by PCR. However, this gene only works when the content of lipopeptide is high, and the activity is not high, so it can be strengthened by the strong constituent promoter.
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