Difference between revisions of "Part:BBa K2924035:Design"

(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
−
The sequence was is codon-optimized for Synechocystis sp. PCC 6803.
+
The sequence is codon-optimized for Synechocystis sp. PCC 6803.
  
 
===References===
 
===References===

Revision as of 12:43, 12 October 2019


mVenus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 630
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 28
    Illegal AgeI site found at 151
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence is codon-optimized for Synechocystis sp. PCC 6803.

References

Kremers, Gert-Jan, et al. "Cyan and yellow super fluorescent proteins with improved brightness, protein folding, and FRET Förster radius." Biochemistry 45.21 (2006): 6570-6580.

Behle, Anna, Pia Saake, and Ilka M. Axmann. "Comparative analysis of inducible promoters in cyanobacteria." bioRxiv (2019): 757948.

https://www.fpbase.org/protein/mvenus/