Difference between revisions of "Part:BBa K2924035:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
 
The sequence was is codon-optimized for Synechocystis sp. PCC 6803.
 
The sequence was is codon-optimized for Synechocystis sp. PCC 6803.
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mVenus is a yellow fluorescent protein derived from gfp and its derivative eYFP, which stem from Aequorea victoria. Its excitation wavelength is at 515 nm and it emits light of 527 nm wavelength1. The protein has a size of 26.9 kDa and is usually found as a monomer.
 
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The sequence was provided codon-optimized for Synechocystis sp. PCC 6803 by Anna Behle.
 
  
 
===References===
 
===References===

Revision as of 12:42, 12 October 2019


mVenus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 630
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 28
    Illegal AgeI site found at 151
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was is codon-optimized for Synechocystis sp. PCC 6803.

References

Kremers, Gert-Jan, et al. "Cyan and yellow super fluorescent proteins with improved brightness, protein folding, and FRET Förster radius." Biochemistry 45.21 (2006): 6570-6580.

Behle, Anna, Pia Saake, and Ilka M. Axmann. "Comparative analysis of inducible promoters in cyanobacteria." bioRxiv (2019): 757948.

https://www.fpbase.org/protein/mvenus/