Difference between revisions of "Part:BBa K2924035:Design"
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===References=== | ===References=== | ||
Kremers, Gert-Jan, et al. "Cyan and yellow super fluorescent proteins with improved brightness, protein folding, and FRET Förster radius." Biochemistry 45.21 (2006): 6570-6580. | Kremers, Gert-Jan, et al. "Cyan and yellow super fluorescent proteins with improved brightness, protein folding, and FRET Förster radius." Biochemistry 45.21 (2006): 6570-6580. | ||
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Behle, Anna, Pia Saake, and Ilka M. Axmann. "Comparative analysis of inducible promoters in cyanobacteria." bioRxiv (2019): 757948. | Behle, Anna, Pia Saake, and Ilka M. Axmann. "Comparative analysis of inducible promoters in cyanobacteria." bioRxiv (2019): 757948. | ||
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https://www.fpbase.org/protein/mvenus/ | https://www.fpbase.org/protein/mvenus/ | ||
Revision as of 12:42, 12 October 2019
mVenus
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 4
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 630
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 28
Illegal AgeI site found at 151 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence was is codon-optimized for Synechocystis sp. PCC 6803.
mVenus is a yellow fluorescent protein derived from gfp and its derivative eYFP, which stem from Aequorea victoria. Its excitation wavelength is at 515 nm and it emits light of 527 nm wavelength1. The protein has a size of 26.9 kDa and is usually found as a monomer. The sequence was provided codon-optimized for Synechocystis sp. PCC 6803 by Anna Behle.
References
Kremers, Gert-Jan, et al. "Cyan and yellow super fluorescent proteins with improved brightness, protein folding, and FRET Förster radius." Biochemistry 45.21 (2006): 6570-6580.
Behle, Anna, Pia Saake, and Ilka M. Axmann. "Comparative analysis of inducible promoters in cyanobacteria." bioRxiv (2019): 757948.