Difference between revisions of "Part:BBa K2938014"
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The plasmid was constructed using gibson assembly, then was incorporated into E.coli BL21 and DH5a using Heat shock, and into serratia marcescens 274 using electroporation. | The plasmid was constructed using gibson assembly, then was incorporated into E.coli BL21 and DH5a using Heat shock, and into serratia marcescens 274 using electroporation. | ||
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| + | ===Characterization=== | ||
| + | Western of Cry4Ba subunit with anti-HA antibody. proving the translation of the toxin subunit on the plasmid. | ||
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| + | [[File:Cry4Ba_Western.png|200px|thumb|left|Western Blot of Bacteria expressing Cry4Ba and deGFP – proof of Cry4Ba expression]] | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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Revision as of 10:00, 12 October 2019
Cry4Ba (HA tag) + deGFP Device
PR1 - RBS - Cry4Ba (HA tag) - RBS - deGFP - T500 Terminator
This plasmid was planned on a pBEST plasmid backbone. It contains larvicidal activity, as it containing toxic subunit of the bacillus thuringiensis israelensis (BTI) pBtoxis plasmid which is toxic to mosquitoes larva.
The plasmid was constructed using gibson assembly, then was incorporated into E.coli BL21 and DH5a using Heat shock, and into serratia marcescens 274 using electroporation.
Characterization
Western of Cry4Ba subunit with anti-HA antibody. proving the translation of the toxin subunit on the plasmid.
