Difference between revisions of "Part:BBa K3102023:Design"
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===Source=== | ===Source=== | ||
| − | + | Neisseria Sicca, sequence found through genebank : https://www.ncbi.nlm.nih.gov/protein/BAJ21448.1 | |
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| + | Promoter (BBa_I719005) , RBS (BBa_B0034) and Terminator (BBa_B0015) are from the iGEM Registry. | ||
===References=== | ===References=== | ||
Revision as of 09:51, 12 October 2019
T7-CAE-ter (secreted CAE)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 124
Illegal NgoMIV site found at 1234
Illegal AgeI site found at 254
Illegal AgeI site found at 341
Illegal AgeI site found at 479
Illegal AgeI site found at 662
Illegal AgeI site found at 710
Illegal AgeI site found at 878
Illegal AgeI site found at 1091 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
CAE:Sequence synthesized and optimized for E.coli on Geneius
Source
Neisseria Sicca, sequence found through genebank : https://www.ncbi.nlm.nih.gov/protein/BAJ21448.1
Promoter (BBa_I719005) , RBS (BBa_B0034) and Terminator (BBa_B0015) are from the iGEM Registry.