Difference between revisions of "Part:BBa K3102038:Design"

(→‎Design Notes)
(→‎Source)
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===Source===
 
===Source===
  
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None
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This sequence comes from Shewanella Oneidensis and was found through Biocyc:
 +
https://biocyc.org/gene?orgid=SONE211586&id=G1GMP-2522#tab=TU
 +
 
 +
Promoter (BBa_I719005), RBS (BBa_B0034) and Terminator (BBa_B0015) are from the iGEM Registry.
  
 
===References===
 
===References===

Revision as of 09:50, 12 October 2019


T7-RBS-ACS-Ter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 966
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We mutated a Cytosine into Guanine at the 1609bp position (C1609T) because of the Esp3I enzyme that we used for our Golden Gate Assembly (GGA) construct. (e.g. BBa_K3102022)

Source

This sequence comes from Shewanella Oneidensis and was found through Biocyc: https://biocyc.org/gene?orgid=SONE211586&id=G1GMP-2522#tab=TU

Promoter (BBa_I719005), RBS (BBa_B0034) and Terminator (BBa_B0015) are from the iGEM Registry.

References