Difference between revisions of "Part:BBa K2938008"

Latest revision as of 09:47, 12 October 2019

P20 with His tag

P20 chaperone with Strep-tag attached in the C- terminal in order to be able to detect the translated protein in western blot (using antibodies against the tag)

Description

p20 is a 20 kDa protein which can be isolated from Bacillus thuringiensis subsp. israelensis (Bti). It is a chaperone, which increases the production of Cry11Aa while expressed, and leads to higher mosquito larvicidal activity [2].

Amplification

PCR amplification of P20 by PCR.

Usage and Biology

The P20 is a chaperone which increases the production of Cry11Aa toxin, increases the larvicidal effect on mosquitos’ larvae, and His-tag is used to detect proteins in western blot using antibodies specific to the tag.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 264
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 264
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 264
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 264
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 264
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 P20 chaperone with Strep-tag attached in the C- terminal in order to be able to detect the translated protein in western blot (using antibodies against the tag)
+===Description===
+p20 is a 20 kDa protein which can be isolated from Bacillus thuringiensis subsp. israelensis (Bti). It is a chaperone, which increases the production of Cry11Aa while expressed, and leads to higher mosquito larvicidal activity [2].
+===Amplification===
+[[File:P20 PCR.jpeg|200px|thumb|left|PCR  amplification of P20 by PCR.]]
 <!-- Add more about the biology of this part here -->