Difference between revisions of "Part:BBa K3102040:Design"
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===Source=== | ===Source=== | ||
| − | + | This sequence comes from Shewanella Oneidensis and was found through Biocyc: | |
| + | https://biocyc.org/gene?orgid=SONE211586&id=G1GMP-721 | ||
| + | |||
| + | Promoter (BBa_I719005) , RBS (BBa_B0034) and Terminator (BBa_B0015) are from the iGEM Registry. | ||
===References=== | ===References=== | ||
Revision as of 09:42, 12 October 2019
T7-RBS-MDH-Ter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 299
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 143
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We mutated an Adenine into Guanine at the 829bp position (A829G) because of the EcoRI enzyme.
Source
This sequence comes from Shewanella Oneidensis and was found through Biocyc: https://biocyc.org/gene?orgid=SONE211586&id=G1GMP-721
Promoter (BBa_I719005) , RBS (BBa_B0034) and Terminator (BBa_B0015) are from the iGEM Registry.