Difference between revisions of "Part:BBa K2938007:Design"

 
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
Gibson assembly
+
Gibson assembly. Tag was added through primers.
 +
Cyt1Aa was isolated from pVE4-ADRC plasmid by PCR.
 +
and was optimized for E.Coli
  
 
===References===
 
===References===

Revision as of 09:37, 12 October 2019


Cyt1Aa With Strep Tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 419
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 419
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 717
    Illegal BamHI site found at 96
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 419
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 419
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Strep-tag fused to C- terminal


Source

Gibson assembly. Tag was added through primers. Cyt1Aa was isolated from pVE4-ADRC plasmid by PCR. and was optimized for E.Coli

References