Difference between revisions of "Part:BBa K2938005:Design"

 
 
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
Gibson assembly
+
Gibson assembly - tag was added through the primers.
 +
Cry11Aa was Isolated from pVE4-ADRC plasmid by PCR.
 +
and optimized for E.Coli
  
 
===References===
 
===References===
 +
[1] V. Khasdan, “Thesis submitted in partial fulfillment : Cloning Combinations of Four Genes from Bacillus thuringiensis,” no. October, 2015.
 +
 +
[2] C. Xu, B. C. Wang, Z. Yu, and M. Sun, “Structural insights into Bacillus thuringiensis Cry, Cyt and parasporin toxins,” Toxins, vol. 6, no. 9. pp. 2732–2770, 01-Sep-2014.
 +
 +
[3] E. Ben-Dov, “Bacillus thuringiensis subsp. israelensis and its dipteran-specific toxins.,” Toxins (Basel)., vol. 6, no. 4, pp. 1222–43, Mar. 2014.

Latest revision as of 09:33, 12 October 2019


Cry11Aa + Strep tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1719
    Illegal SpeI site found at 831
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1719
    Illegal SpeI site found at 831
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1719
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1719
    Illegal SpeI site found at 831
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1719
    Illegal SpeI site found at 831
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Strep-tag fused to N- terminal


Source

Gibson assembly - tag was added through the primers. Cry11Aa was Isolated from pVE4-ADRC plasmid by PCR. and optimized for E.Coli

References

[1] V. Khasdan, “Thesis submitted in partial fulfillment : Cloning Combinations of Four Genes from Bacillus thuringiensis,” no. October, 2015.

[2] C. Xu, B. C. Wang, Z. Yu, and M. Sun, “Structural insights into Bacillus thuringiensis Cry, Cyt and parasporin toxins,” Toxins, vol. 6, no. 9. pp. 2732–2770, 01-Sep-2014.

[3] E. Ben-Dov, “Bacillus thuringiensis subsp. israelensis and its dipteran-specific toxins.,” Toxins (Basel)., vol. 6, no. 4, pp. 1222–43, Mar. 2014.