Difference between revisions of "Part:BBa K3102032:Design"

 
(→‎Design Notes)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
None
 
−
 
  
 +
We mutated a Thymine into Cysteine at the 844bp position (T844C) because of the PstI enzyme
  
 
===Source===
 
===Source===

Revision as of 09:30, 12 October 2019


T7-RBS-ZWF-Ter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 810
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 810
    Illegal NotI site found at 691
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1367
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 810
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 810
    Illegal NgoMIV site found at 83
    Illegal NgoMIV site found at 800
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We mutated a Thymine into Cysteine at the 844bp position (T844C) because of the PstI enzyme

Source

None

References