Difference between revisions of "Part:BBa K3102028"

Revision as of 09:08, 12 October 2019

T7-RBS-ACS operon-Ter

This T7-ACS operon-ter composite is composed of ACS operon part (BBa_K3102013) composed of three genes: actP (Transports acetate) acs (catalyzes the conversion of acetate into acetyl-CoA) and yfcH (epimerase)[Gimenez R et al., 2003]. This part is useful to increase the overexpression of a ACS and transport of the acetate. This composite is useful to increase the overexpression of the operon and thus, the uptake of acetate into the cell to metabolize it into other substrates.

This constructs is enriched with primer, T7 promoters, RBS and terminator.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 408
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 837
Illegal AgeI site found at 2428
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 3702

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3102028 short</partinfo>
-This T7-ACS operon-ter composite composes of ACS operon part (BBa_K3102013) is used in order to secrete both CAE and EG1 proteins by E.coli.
+This T7-ACS operon-ter composite is composed of ACS operon part (BBa_K3102013) composed of three genes: actP (Transports acetate) acs (catalyzes the conversion of acetate into acetyl-CoA) and yfcH (epimerase)[Gimenez R et al., 2003]. This part is useful to increase the overexpression of a ACS and transport of the acetate.
-actP (Transports acetate) acs (catalyzes the conversion of acetate into acetyl-CoA) yfcH
+This composite is useful to increase the overexpression of the operon and thus, the uptake of acetate into the cell to metabolize it into other substrates.
 This constructs is enriched with primer, T7 promoters, RBS and terminator.