Difference between revisions of "Part:BBa K2963020:Design"
Line 14: | Line 14: | ||
===Source=== | ===Source=== | ||
− | Bacillus | + | Derived from Bacillus subtilis. |
===References=== | ===References=== |
Latest revision as of 07:02, 12 October 2019
pgsA - encoding a submit of poly-γ-glutamic acid synthetase
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 364
Illegal PstI site found at 650 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 364
Illegal PstI site found at 650 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 364
Illegal PstI site found at 650 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 364
Illegal PstI site found at 650
Illegal AgeI site found at 1008 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
When we express the CapA protein heterologously in corynebacterium glutamicum, we add Ptac promoter and a short RBS, which can be recognized by corynebacterium glutamicum. And simultaneously, we add T7 terminater used for stopping transcription in time.
Source
Derived from Bacillus subtilis.