Difference between revisions of "Part:BBa K2963019:Design"
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===Source=== | ===Source=== | ||
| − | Bacillus | + | Derived from Bacillus subtilis. |
===References=== | ===References=== | ||
Latest revision as of 07:01, 12 October 2019
pgsC - encoding a subunit of poly-γ-glutamic acid synthetase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 81
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
When we express the PgsC protein heterologously in corynebacterium glutamicum, we add Ptac promoter and a short RBS, which can be recognized by corynebacterium glutamicum. And simultaneously, we add T7 terminater used for stopping transcription in time.
Source
Derived from Bacillus subtilis.