Difference between revisions of "Part:BBa K2923000"

 
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<partinfo>BBa_K2923000 short</partinfo>
 
<partinfo>BBa_K2923000 short</partinfo>
  
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Our bacterial triple hybrid (BH3) construct is composed of two fusion proteins with MS2 and PP7 coat protein and RNA. This part is composed of two MS2 (from Escherichia phage MS2) and two PP7 (Pseudomonas phage PP7) stem-loops. MS2 and PP7 are able to recognize specifically MS2 and PP7 coat proteins for linked all parts of the contract.  
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Our bacterial three-hybrid (BH3) construct (inspired by Berry and Hochschild, 2018) is composed of two fusion proteins with MS2 and PP7 coat protein and RNA. This present part is composed of two MS2 (from Escherichia phage MS2) and two PP7 (Pseudomonas phage PP7) stem-loops. MS2 and PP7 are able to recognize specifically MS2 and PP7 coat proteins for linked all parts of the construct.  
 
In our project MS2_MS2-PP7_PP7 RNA is used as a positive control for the BH3 system.  
 
In our project MS2_MS2-PP7_PP7 RNA is used as a positive control for the BH3 system.  
 
The sequence between the two MS2 and two PP7 contain 6 differents restrictions sites: PstI, AvaI, XmaI, BsoBI, TspMI, and SmaI.  
 
The sequence between the two MS2 and two PP7 contain 6 differents restrictions sites: PstI, AvaI, XmaI, BsoBI, TspMI, and SmaI.  

Revision as of 05:46, 12 October 2019


MS2_MS2-PP7_PP7 RNA

Our bacterial three-hybrid (BH3) construct (inspired by Berry and Hochschild, 2018) is composed of two fusion proteins with MS2 and PP7 coat protein and RNA. This present part is composed of two MS2 (from Escherichia phage MS2) and two PP7 (Pseudomonas phage PP7) stem-loops. MS2 and PP7 are able to recognize specifically MS2 and PP7 coat proteins for linked all parts of the construct. In our project MS2_MS2-PP7_PP7 RNA is used as a positive control for the BH3 system. The sequence between the two MS2 and two PP7 contain 6 differents restrictions sites: PstI, AvaI, XmaI, BsoBI, TspMI, and SmaI. This part permits to create a new RNA sequence bordered by MS2 and PP7 stem-loops.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 37
    Illegal PstI site found at 25
    Illegal PstI site found at 64
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 25
    Illegal PstI site found at 64
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 37
    Illegal PstI site found at 25
    Illegal PstI site found at 64
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 37
    Illegal PstI site found at 25
    Illegal PstI site found at 64
  • 1000
    COMPATIBLE WITH RFC[1000]