Difference between revisions of "Part:BBa K3174008"
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This is a redesigned version of the Super Yellow Fluorescent Protein 2 (BBa_K864100) that has had a region that was prone to insertion element mutations altered. This removal of a mutation hotspot resulted in the expression of SYFP2 in culture being maintained for a longer period of time than the original plasmid. | This is a redesigned version of the Super Yellow Fluorescent Protein 2 (BBa_K864100) that has had a region that was prone to insertion element mutations altered. This removal of a mutation hotspot resulted in the expression of SYFP2 in culture being maintained for a longer period of time than the original plasmid. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 15:04, 11 October 2019
SYFP2 coding sequence with IS hotspot removed
This is a redesigned version of the Super Yellow Fluorescent Protein 2 (BBa_K864100) that has had a region that was prone to insertion element mutations altered. This removal of a mutation hotspot resulted in the expression of SYFP2 in culture being maintained for a longer period of time than the original plasmid.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]