Difference between revisions of "Part:BBa K2934008"
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===Design=== | ===Design=== | ||
− | [[ | + | [[file:Synthetic_circuit.png|400px|thumb|right|figure 1: our design of the Honey Circuit, made by iGEM Technion 2019 team BeeFree]] |
This part is made to produce the enzyme Glucose Oxidase (GOx) by <em>Bacillus subtilis</em> in a regulated manner. The part contains the Lac operon inhibitor (BBa_K143033), under the regulation of KatA promoter (BBa_K2934002). At growing concentrations of hydrogen peroxide, the suppression of KatA promoter is reduced, bringing to the production of LacI, which will suppress the production of GOx (under the regulation of pLac BBa_K143015). The GOx enzyme production in this circuit has negative feedback. | This part is made to produce the enzyme Glucose Oxidase (GOx) by <em>Bacillus subtilis</em> in a regulated manner. The part contains the Lac operon inhibitor (BBa_K143033), under the regulation of KatA promoter (BBa_K2934002). At growing concentrations of hydrogen peroxide, the suppression of KatA promoter is reduced, bringing to the production of LacI, which will suppress the production of GOx (under the regulation of pLac BBa_K143015). The GOx enzyme production in this circuit has negative feedback. | ||
Revision as of 13:57, 11 October 2019
pKatA-LacI-pLac-Glucose Oxidase-Histag for B. subtilis
Design
This part is made to produce the enzyme Glucose Oxidase (GOx) by Bacillus subtilis in a regulated manner. The part contains the Lac operon inhibitor (BBa_K143033), under the regulation of KatA promoter (BBa_K2934002). At growing concentrations of hydrogen peroxide, the suppression of KatA promoter is reduced, bringing to the production of LacI, which will suppress the production of GOx (under the regulation of pLac BBa_K143015). The GOx enzyme production in this circuit has negative feedback.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1268
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1564
Illegal BsaI site found at 1895
Illegal BsaI.rc site found at 1841
Illegal BsaI.rc site found at 2666