Difference between revisions of "Part:BBa K2996005"
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<partinfo>BBa_K2996005 short</partinfo> | <partinfo>BBa_K2996005 short</partinfo> | ||
| − | Tetracycline-Controlled transcriptional activation is a method of inducible gene expression where transcription is reversibly turned on or off in the presence of the antibiotic tetracycline, with a final | + | Tetracycline-Controlled transcriptional activation is a method of inducible gene expression where transcription is reversibly turned on or off in the presence of the antibiotic tetracycline, with a final concentration at 100ug/ml adding to E. coli at early expotational stage. |
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| + | Demonstrated by our experiments, addition of tetracycline is the only signal needed to trigger spacer acquisition, thus accomplishing information storage. | ||
| + | 诶这个要改表达 | ||
Latest revision as of 13:06, 11 October 2019
PLtetO-1 promoter
Tetracycline-Controlled transcriptional activation is a method of inducible gene expression where transcription is reversibly turned on or off in the presence of the antibiotic tetracycline, with a final concentration at 100ug/ml adding to E. coli at early expotational stage.
Demonstrated by our experiments, addition of tetracycline is the only signal needed to trigger spacer acquisition, thus accomplishing information storage. 诶这个要改表达
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]