Difference between revisions of "Part:BBa K3257044"
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| − | Cre recombinase is responsible for recombination of reverse transcribed cDNA, which is also one of the most important processes in our mutation library construction. We have expressed Cre recombinase in E.coli and proved its ability of gene cleavage when there are lox sites present. | + | Cre recombinase is responsible for the recombination of reverse-transcribed cDNA, which is also one of the most important processes in our mutation library construction. We have expressed Cre recombinase in E.coli and proved its ability of gene cleavage when there are lox sites present. |
| + | [[File:ILacI+IPTG.png|none|500px|thumb|'''Figure 2. The induction level of EGFP under different repressors and promoters.''' The induction level is calculated by dividing the fluorescence level after 9 h of induction by 1 h afterward. The fluorescence level is quantified as in Fig. 1. oLacI stands for the wildtype LacI, iLacI stands for our improved version of LacI. +LacO indicates that the promoter constitutes a LacO sequence. t-test analysis shows that the induction level of iLacI is significantly higher than oLacI, *** indicates that p=0.0002.]] | ||
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Revision as of 12:12, 11 October 2019
Cre Recombinase
Cre recombinase is responsible for the recombination of reverse-transcribed cDNA, which is also one of the most important processes in our mutation library construction. We have expressed Cre recombinase in E.coli and proved its ability of gene cleavage when there are lox sites present.
Figure 2. The induction level of EGFP under different repressors and promoters. The induction level is calculated by dividing the fluorescence level after 9 h of induction by 1 h afterward. The fluorescence level is quantified as in Fig. 1. oLacI stands for the wildtype LacI, iLacI stands for our improved version of LacI. +LacO indicates that the promoter constitutes a LacO sequence. t-test analysis shows that the induction level of iLacI is significantly higher than oLacI, *** indicates that p=0.0002.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 356
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 42
- 1000COMPATIBLE WITH RFC[1000]