Difference between revisions of "Part:BBa K2936013"

 
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===Usage and Biology===
 
===Usage and Biology===
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Enzymes expressed by gfa, fghA and flhA gradually degraded formaldehyde into formic acid.
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 11:40, 11 October 2019


Formaldehyde degradation system

Formaldehyde reaction of formic acid need Glutathione-dependent Formaldehyde-activating Enzyme (GFA), Glutathione-dependent formaldehyde dehydrogenase (GS-FDH) and S-formylglut-athione hydrolase (FGH) which come from Paracoccus denitrificans (p. denitrificans) catalyst. GFA can act as a glutathione (GSH) carrier to catalyze the reaction of GSH and CH2O to form s-hydroxy-methylglutathione (HMG)[1], GS-FDH can catalyze the oxidation of HMG dehydrogenation to form s-formylglutathione, and FGH can regenerate GSH.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 904
    Illegal PstI site found at 1384
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 904
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 1384
    Illegal NotI site found at 1745
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 904
    Illegal BglII site found at 1265
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 904
    Illegal PstI site found at 1384
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 904
    Illegal PstI site found at 1384
    Illegal NgoMIV site found at 853
    Illegal NgoMIV site found at 1049
    Illegal NgoMIV site found at 1402
    Illegal NgoMIV site found at 1662
    Illegal NgoMIV site found at 2138
    Illegal NgoMIV site found at 2249
  • 1000
    COMPATIBLE WITH RFC[1000]