Difference between revisions of "Part:BBa K2934001:Design"

Revision as of 09:48, 11 October 2019

Invertase-Histag A. niger optimized for B. subtilis

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 863
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1168
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 469
Illegal BsaI.rc site found at 811
Illegal SapI.rc site found at 1071

Design Notes

The sequence is based on the A. niger Invertase amino acid sequence and codon optimization for B. subtilis, using codon usage tables [2] [3]. The linker is based on the pBE-S commercial plasmid by TaKaRa [4] that we used for protein secretion from B. subtilis. We changed two bases at the locations 1818 and 1833 (without changing the amino acids) to removed unwanted PstI and XbaI restriction sites.

Source

The sequence is based on the Invertase amino acid sequence [1] and is optimized for B. subtilis.

References

[1] L. M. Boddy, T. Berges, C. Barreau, M. H. Vainstein, M. J. Dobson, D. J. Ballance, j. E Peberdy. 1993. Purification and characterisation of an Aspergillus niger invertase and its DNA sequence, Current Genetics.

[2] https://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=1423

[3] http://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=224308&aa=1&style=N

@@ Line 7: / Line 7: @@
 ===Design Notes===
-The sequence is based on the A. niger Invertase amino acid sequence and codon optimization for B. subtilis, using codon usage tables [2] [3].
+The sequence is based on the A. niger Invertase amino acid sequence and codon optimization for B. subtilis, using codon usage tables [2] [3]. The linker is based on the pBE-S commercial plasmid by TaKaRa [4] that we used for protein secretion from B. subtilis. We changed two bases at the locations 1818 and 1833 (without changing the amino acids) to removed unwanted PstI and XbaI restriction sites.
 ===Source===