Difference between revisions of "Part:BBa K2934000"

Revision as of 09:33, 11 October 2019

Glucose Oxidase-Histag A. niger optimized for B. subtilis

Glucose Oxidase gene is coding for the Glucose Oxidase (GOx) enzyme, which catalyzes the oxidation of glucose to D-glucono-lacton and release of hydrogen peroxide. The sequence includes histag (6xHis), connected with seven amino acids linker protein at the N terminal of the protein. The enzyme origin is from A. niger and is optimized for B. subtilis.

Usage and Biology

figure 1: the enzymatic reaction of glucose oxidase

Glucose oxidase (GOx) catalyzes the oxidation of glucose to using molecular oxygen to create hydrogen peroxide and D-glucono lactone. GOx requires the cofactor flavin adenine dinucleotide (FAD) that acts as an electron acceptor in a redox reaction. It is reduced to FADH2, then it is oxidized by molecular oxygen, which is then reduced to hydrogen peroxide [1].

References

[1] Frederick KR, Tung J, Richard S. Emerick F, Chamberlain SH, Vasavada A, Steven R, Chakraborty S, Schopter LM, Schopter LM, Massey V. 1990. Glucose Oxidase from Aspergillus niger. J Biol Chem 265:3793–3802.

Sequence and Features