Difference between revisions of "Part:BBa K2996002:Design"
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===References=== | ===References=== | ||
1.Amlinger L , Hoekzema M , Wagner E G H , et al. Fluorescent CRISPR Adaptation Reporter for rapid quantification of spacer acquisition[J]. Scientific Reports, 2017, 7(1):10392. | 1.Amlinger L , Hoekzema M , Wagner E G H , et al. Fluorescent CRISPR Adaptation Reporter for rapid quantification of spacer acquisition[J]. Scientific Reports, 2017, 7(1):10392. | ||
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2.Díez-Villase?or, César, Guzmán, Noemí M, Almendros, Cristóbal, et al. CRISPR-spacer integration reporter plasmids reveal distinct genuine acquisition specificities among CRISPR-Cas I-E variants of Escherichia coli, [J]. RNA Biology, 2013, 10(5):792-802. | 2.Díez-Villase?or, César, Guzmán, Noemí M, Almendros, Cristóbal, et al. CRISPR-spacer integration reporter plasmids reveal distinct genuine acquisition specificities among CRISPR-Cas I-E variants of Escherichia coli, [J]. RNA Biology, 2013, 10(5):792-802. | ||
Latest revision as of 00:48, 11 October 2019
minimal CRISPR array-A
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The structure of RSRL sequence is complicated and it is relatively short, we suggest direct synthesis when applying this biobrick. Also, be careful with the number of necleotides you are adding, since this device only works when the reading frame can be precisely regulated.
Source
RSRL cassette contains 69 bp of the leader2.1 and the adjacent CRISPR-spacer-CRISPR amplified from E. coli K12 str. MG.1655.
References
1.Amlinger L , Hoekzema M , Wagner E G H , et al. Fluorescent CRISPR Adaptation Reporter for rapid quantification of spacer acquisition[J]. Scientific Reports, 2017, 7(1):10392.
2.Díez-Villase?or, César, Guzmán, Noemí M, Almendros, Cristóbal, et al. CRISPR-spacer integration reporter plasmids reveal distinct genuine acquisition specificities among CRISPR-Cas I-E variants of Escherichia coli, [J]. RNA Biology, 2013, 10(5):792-802.